Analysis Note

ControlA431, HeLa, and Jurkat cell lysates

Application

Research CategoryApoptosis & Cancer

Detect Smad using this mouse monoclonal antibody, Anti-SMAD6 Antibody & clone 5H3 validated for use in western blotting.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SMAD6 is the gene name for a protein that is a homolog of the fly gene Mothers against decapentaplegic, and in humans it is called Mothers against decapentaplegic homolog 6 (MAD homolog 6). Other names include SMAD family member 6 and Mothers against DPP homolog 6 and another gene name of MADH6. By whatever name, the protein is a transcription factor that binds to regulatory elements within the nucleus and mediates transcription. The effect is to act as a mediator of TGF-beta and BMP mediated anti-inflammatory signaling activity. SMAD6 suppresses IL1R-TLR signaling, thus preventing NF-kappa B activation and activation of proinflammatory genes. SMAD6 interacts with numerous other intracellular proteins including NEDD4L, STAMBP, PRKK, RNF111, and AXIN1. SMAD6 is expressed in a wide variety of tissues with higher levels being seen in lung and cardiac tissues. Recently SMAD6 has been shown to promote neuronal differentiation by inhibiting the Wnt/beta catenin signaling pathway in addition to inhibiting the BMP signaling. Finally in cancer SMAD6 via its suppression of TGF-beta induced growth inhibition can potentiate the transformed phenotype. Moreover SMAD6 is often over expressed in many cancers, which suppresses G0-G1 arrest and apoptosis which might otherwise normally occur, thus leaving SMAD6 playing a critical role in supporting cancer cell growth and survival. EMD-Millipore's Anti-SMAD6 mouse monoclonal antibody has been tested in western blot against human A431, HeLa, and Jurkat cell lysates.

Immunogen

Purified recombinant fragment of human SMAD6 expressed in E. Coli.

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in A431, HeLa, and Jurkat cell lysates.

Western Blot Analysis: A 1:500-2000 dilution of this antibody detected SMAD6 in A431, HeLa, and Jurkat cell lysates.

Optimal working dilutions must be determined by end user.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~55 kDa observed